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rabbit anti human bcl 2  (Proteintech)


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    Structured Review

    Proteintech rabbit anti human bcl 2
    Rabbit Anti Human Bcl 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 4734 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+bcl+2/BCL2+Antibody/pmc12804270-91-0-14
    Average 96 stars, based on 4734 article reviews
    rabbit anti human bcl 2 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: Nanoparticle-Mediated Co-Delivery of Notch-1 Antibodies and ABT-737 as a Potent Treatment Strategy for Triple-Negative Breast Cancer
    Article Snippet: After removing membrane debris through centrifugation, the extracted protein was quantified using a DC Protein Assay (BioRad), and 10 μ g of protein was separated on 4–12% bis-tris gels at 135 V for 60 min. Then, the protein was transferred to a 0.2 μ m nitrocellulose membrane for 10 min using a Power Blotter System (Invitrogen). .. The membrane was subsequently blocked for 60 min in 5% milk in tris buffered saline with 0.1% Tween-20 (TBS-T) and then incubated with rabbit anti-human Bcl-2 (ProteinTech; 1:1000), cleaved Notch-1 (Cell Signaling Technology; 1:250), and Hes1 antibodies (Cell Signaling Technology; 1:500) in 5% milk in TBS-T overnight at 4 °C. .. They were subsequently incubated in the Click-iT reaction cocktail, washed in PBS, and examined on an Acea Novocyte 2060 flow cytometer with the FITC (excitation, 488 nm; emission, 530/30 nm) channel.

    Article Title: Dual Regulation of miR-34a and Notch Signaling in Triple-Negative Breast Cancer by Antibody/miRNA Nanocarriers
    Article Snippet: After removing membrane debris through centrifugation, the extracted protein was quantified using a DC (detergent compatible) protein assay (Bio-Rad), and 10 μg of protein was separated on 4%–12% Bis-Tris gels at 135 V for 60 min. Then, the protein was transferred to a 0.2-μm nitrocellulose membrane for 10 min using a power blotter system (Invitrogen). .. The membrane was subsequently blocked for 60 min in 5% milk in Tris-buffered saline with 0.1% Tween 20 (TBS-T) and then incubated with rabbit anti-human Bcl-2 (ProteinTech; 1:1,000), CCND1 (Cell Signaling Technology; 1:250), Notch1 (Cell Signaling Technology; 1:500), and Hes1 (Cell Signaling Technology; 1:500) antibodies in 5% milk in TBS-T overnight at 4°C. .. Mouse anti-human α-tubulin antibody (Cell Signaling Technology; 1:20,000) was used as the normalization control.

    Saline:

    Article Title: Nanoparticle-Mediated Co-Delivery of Notch-1 Antibodies and ABT-737 as a Potent Treatment Strategy for Triple-Negative Breast Cancer
    Article Snippet: After removing membrane debris through centrifugation, the extracted protein was quantified using a DC Protein Assay (BioRad), and 10 μ g of protein was separated on 4–12% bis-tris gels at 135 V for 60 min. Then, the protein was transferred to a 0.2 μ m nitrocellulose membrane for 10 min using a Power Blotter System (Invitrogen). .. The membrane was subsequently blocked for 60 min in 5% milk in tris buffered saline with 0.1% Tween-20 (TBS-T) and then incubated with rabbit anti-human Bcl-2 (ProteinTech; 1:1000), cleaved Notch-1 (Cell Signaling Technology; 1:250), and Hes1 antibodies (Cell Signaling Technology; 1:500) in 5% milk in TBS-T overnight at 4 °C. .. They were subsequently incubated in the Click-iT reaction cocktail, washed in PBS, and examined on an Acea Novocyte 2060 flow cytometer with the FITC (excitation, 488 nm; emission, 530/30 nm) channel.

    Article Title: Dual Regulation of miR-34a and Notch Signaling in Triple-Negative Breast Cancer by Antibody/miRNA Nanocarriers
    Article Snippet: After removing membrane debris through centrifugation, the extracted protein was quantified using a DC (detergent compatible) protein assay (Bio-Rad), and 10 μg of protein was separated on 4%–12% Bis-Tris gels at 135 V for 60 min. Then, the protein was transferred to a 0.2-μm nitrocellulose membrane for 10 min using a power blotter system (Invitrogen). .. The membrane was subsequently blocked for 60 min in 5% milk in Tris-buffered saline with 0.1% Tween 20 (TBS-T) and then incubated with rabbit anti-human Bcl-2 (ProteinTech; 1:1,000), CCND1 (Cell Signaling Technology; 1:250), Notch1 (Cell Signaling Technology; 1:500), and Hes1 (Cell Signaling Technology; 1:500) antibodies in 5% milk in TBS-T overnight at 4°C. .. Mouse anti-human α-tubulin antibody (Cell Signaling Technology; 1:20,000) was used as the normalization control.

    Incubation:

    Article Title: Nanoparticle-Mediated Co-Delivery of Notch-1 Antibodies and ABT-737 as a Potent Treatment Strategy for Triple-Negative Breast Cancer
    Article Snippet: After removing membrane debris through centrifugation, the extracted protein was quantified using a DC Protein Assay (BioRad), and 10 μ g of protein was separated on 4–12% bis-tris gels at 135 V for 60 min. Then, the protein was transferred to a 0.2 μ m nitrocellulose membrane for 10 min using a Power Blotter System (Invitrogen). .. The membrane was subsequently blocked for 60 min in 5% milk in tris buffered saline with 0.1% Tween-20 (TBS-T) and then incubated with rabbit anti-human Bcl-2 (ProteinTech; 1:1000), cleaved Notch-1 (Cell Signaling Technology; 1:250), and Hes1 antibodies (Cell Signaling Technology; 1:500) in 5% milk in TBS-T overnight at 4 °C. .. They were subsequently incubated in the Click-iT reaction cocktail, washed in PBS, and examined on an Acea Novocyte 2060 flow cytometer with the FITC (excitation, 488 nm; emission, 530/30 nm) channel.

    Article Title: Layer-by-layer assembled PLGA nanoparticles carrying miR-34a cargo inhibit the proliferation and cell cycle progression of triple-negative breast cancer cells
    Article Snippet: .. After blocking, membranes were incubated with rabbit anti-human CCND-1 (Cell Signaling; 1:500 dilution) or rabbit anti-human Bcl-2 (Proteintech; 1:2000 dilution) antibodies in TBST with 5% milk overnight at 4°C. .. Then, membranes were washed thrice in TBST and incubated with horseradish peroxidase (HRP)-mouse anti-rabbit IgG (Santa Cruz) diluted to 1:25000 in TBST with 5% milk for 1 hr at room temperature.

    Article Title: Dual Regulation of miR-34a and Notch Signaling in Triple-Negative Breast Cancer by Antibody/miRNA Nanocarriers
    Article Snippet: After removing membrane debris through centrifugation, the extracted protein was quantified using a DC (detergent compatible) protein assay (Bio-Rad), and 10 μg of protein was separated on 4%–12% Bis-Tris gels at 135 V for 60 min. Then, the protein was transferred to a 0.2-μm nitrocellulose membrane for 10 min using a power blotter system (Invitrogen). .. The membrane was subsequently blocked for 60 min in 5% milk in Tris-buffered saline with 0.1% Tween 20 (TBS-T) and then incubated with rabbit anti-human Bcl-2 (ProteinTech; 1:1,000), CCND1 (Cell Signaling Technology; 1:250), Notch1 (Cell Signaling Technology; 1:500), and Hes1 (Cell Signaling Technology; 1:500) antibodies in 5% milk in TBS-T overnight at 4°C. .. Mouse anti-human α-tubulin antibody (Cell Signaling Technology; 1:20,000) was used as the normalization control.

    other:

    Article Title: Layer-by-layer assembled PLGA nanoparticles carrying miR-34a cargo inhibit the proliferation and cell cycle progression of triple-negative breast cancer cells
    Article Snippet: Protein concentration was determined using a DC Protein Assay (BioRad) and then 30 μg of protein was separated on 4–12% bis-tris gels at 135 V for 60 min. Next, the protein was transferred to 0.2 μm nitrocellulose membranes for 10 min using the Pierce Power System (Thermo Scientific).

    Blocking Assay:

    Article Title: Layer-by-layer assembled PLGA nanoparticles carrying miR-34a cargo inhibit the proliferation and cell cycle progression of triple-negative breast cancer cells
    Article Snippet: .. After blocking, membranes were incubated with rabbit anti-human CCND-1 (Cell Signaling; 1:500 dilution) or rabbit anti-human Bcl-2 (Proteintech; 1:2000 dilution) antibodies in TBST with 5% milk overnight at 4°C. .. Then, membranes were washed thrice in TBST and incubated with horseradish peroxidase (HRP)-mouse anti-rabbit IgG (Santa Cruz) diluted to 1:25000 in TBST with 5% milk for 1 hr at room temperature.



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